DoE provides funds for human genome sequencing

Sequencing the Human Genome

Human whole-genome shotgun sequencing.

Large-scale sequencing of the human genome is now under way (Boguski et al. 1996; Marshall and Pennisi 1996). Although at the beginning of the Genome Project, many doubted the scientific value of sequencing the entire human genome, these doubts have evaporated almost entirely (Gibbs 1995; Olson 1995). Primary reasons for generating the human genomic sequence are listed in Table 1. The approach ...

MBD-isolated Genome Sequencing provides a high-throughput and comprehensive survey of DNA methylation in the human genome

DNA methylation is an epigenetic modification involved in both normal developmental processes and disease states through the modulation of gene expression and the maintenance of genomic organization. Conventional methods of DNA methylation analysis, such as bisulfite sequencing, methylation sensitive restriction enzyme digestion and array-based detection techniques, have major limitations that ...

Organism Sequencing , Annotation , and Comparative Genomics 1 U . S . DOE Joint Genome Institute Microbial Sequencing : Genomes to Life Projects

The US DOE Joint Genome Institute (JGI) sequences microbial and metagenomic projects through three main programs: DOE Microbial Genome Program (MGP), JGI Community Sequencing Program (CSP) and DOE Genomes to Life Program (GTL). The principle goal of the MGP is to fund sequencing projects related to DOE interests, the principle goal of the CSP is to fund sequencing projects from a broad range of...

On the sequencing of the human genome.

Two recent papers using different approaches reported draft sequences of the human genome. The international Human Genome Project (HGP) used the hierarchical shotgun approach, whereas Celera Genomics adopted the whole-genome shotgun (WGS) approach. Here, we analyze whether the latter paper provides a meaningful test of the WGS approach on a mammalian genome. In the Celera paper, the authors did...